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pbluescript ii sk1 vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii sk1 vector
    Pbluescript Ii Sk1 Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk1+vector/pm19326446-58-34-38
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk1 vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Amphioxus Evx genes: implications for the evolution of the Midbrain-Hindbrain Boundary and the chordate tailbud.
    Article Snippet: .. The 110bp band was cloned into pBluescript SK1 vector (Stratagene) and 50 clones Copyright © 2001 by Academic Press. .. All right sequenced automatically (ABI Perkin Elmer).

    Article Title: Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
    Article Snippet: .. The PCR roducts (about 380 bp) were separated on a 3% Nusieve gel, igested with BamHI and HindIII, and cloned between the BamHI nd HindIII sites of pBluescript SK1 vector (Stratagene). equencing and Library Screening Purified plasmid DNAs containing PCR products were seuenced using an automatic DNA sequencer, DSQ1000L (Shiadzu). ..

    Article Title: An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.
    Article Snippet: DNA Cloning and Sequence Analysis—PCR products were separated by agarose gel electrophoresis and recovered with DEAE-cellulose membranes (NA-45, Schleicher and Schuell, Dassel, Germany). .. The fragments were blunt-ended with Escherichia coli DNA polymerase I (Takara Syuzo, Kyoto, Japan), cloned into an EcoRV-digested pBluescript SK1 vector (Stratagene, La Jolla, CA), and introduced into E. coli DH5. .. The recombinant plasmids were propagated and sequenced using the DyeDeoxy terminator cycle sequencing kit and model 373A DNA sequencer (Applied Biosystems).

    Article Title: Identification of an RNA Binding Specificity for the Potential Splicing Factor TLS
    Article Snippet: .. After the last round of selection, the resultant BamHI-EcoRI DNA was cloned into pBluescript SK1 vector (Stratagene) and sequenced. ..

    Plasmid Preparation:

    Article Title: Amphioxus Evx genes: implications for the evolution of the Midbrain-Hindbrain Boundary and the chordate tailbud.
    Article Snippet: .. The 110bp band was cloned into pBluescript SK1 vector (Stratagene) and 50 clones Copyright © 2001 by Academic Press. .. All right sequenced automatically (ABI Perkin Elmer).

    Article Title: Energetic and Functional Contribution of Residues in the Core Binding Factor β (CBFβ) Subunit to Heterodimerization with CBFα
    Article Snippet: .. This fragment was then subcloned into the pBluescript SK1 vector (Stratagene) between the XhoI and HindIII sites. .. Site-directed mutagenesis was performed on this plasmid using the Stratagene QuikChange mutagenesis kit following the manufacturer’s protocols.

    Article Title: Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
    Article Snippet: .. The PCR roducts (about 380 bp) were separated on a 3% Nusieve gel, igested with BamHI and HindIII, and cloned between the BamHI nd HindIII sites of pBluescript SK1 vector (Stratagene). equencing and Library Screening Purified plasmid DNAs containing PCR products were seuenced using an automatic DNA sequencer, DSQ1000L (Shiadzu). ..

    Article Title: An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.
    Article Snippet: DNA Cloning and Sequence Analysis—PCR products were separated by agarose gel electrophoresis and recovered with DEAE-cellulose membranes (NA-45, Schleicher and Schuell, Dassel, Germany). .. The fragments were blunt-ended with Escherichia coli DNA polymerase I (Takara Syuzo, Kyoto, Japan), cloned into an EcoRV-digested pBluescript SK1 vector (Stratagene, La Jolla, CA), and introduced into E. coli DH5. .. The recombinant plasmids were propagated and sequenced using the DyeDeoxy terminator cycle sequencing kit and model 373A DNA sequencer (Applied Biosystems).

    Article Title: EMK protein kinase-null mice: dwarfism and hypofertility associated with alterations in the somatotrope and prolactin pathways.
    Article Snippet: A custom-made (Clontech, USA) l dash II genomic ibrary was constructed from genomic DNA of “38” transgenic ice and hybridized with a b-geo probe. .. Phage DNA was subcloned n pbluescript SK1 vector (Stratagene, France). ..

    Article Title: Identification of an RNA Binding Specificity for the Potential Splicing Factor TLS
    Article Snippet: .. After the last round of selection, the resultant BamHI-EcoRI DNA was cloned into pBluescript SK1 vector (Stratagene) and sequenced. ..

    Article Title: A Silencer Element in the Regulatory Region of Glutamine Synthetase Controls Cell Type-specific Repression of Gene Induction by Glucocorticoids
    Article Snippet: .. This paper is available on line at http://www.jbc.org 11399 (11) into the pBluescript SK1 vector (Stratagene). ..

    Polymerase Chain Reaction:

    Article Title: Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
    Article Snippet: .. The PCR roducts (about 380 bp) were separated on a 3% Nusieve gel, igested with BamHI and HindIII, and cloned between the BamHI nd HindIII sites of pBluescript SK1 vector (Stratagene). equencing and Library Screening Purified plasmid DNAs containing PCR products were seuenced using an automatic DNA sequencer, DSQ1000L (Shiadzu). ..

    Library Screening:

    Article Title: Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
    Article Snippet: .. The PCR roducts (about 380 bp) were separated on a 3% Nusieve gel, igested with BamHI and HindIII, and cloned between the BamHI nd HindIII sites of pBluescript SK1 vector (Stratagene). equencing and Library Screening Purified plasmid DNAs containing PCR products were seuenced using an automatic DNA sequencer, DSQ1000L (Shiadzu). ..

    Purification:

    Article Title: Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
    Article Snippet: .. The PCR roducts (about 380 bp) were separated on a 3% Nusieve gel, igested with BamHI and HindIII, and cloned between the BamHI nd HindIII sites of pBluescript SK1 vector (Stratagene). equencing and Library Screening Purified plasmid DNAs containing PCR products were seuenced using an automatic DNA sequencer, DSQ1000L (Shiadzu). ..

    Selection:

    Article Title: Identification of an RNA Binding Specificity for the Potential Splicing Factor TLS
    Article Snippet: .. After the last round of selection, the resultant BamHI-EcoRI DNA was cloned into pBluescript SK1 vector (Stratagene) and sequenced. ..



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